Earticle

초록

영어

The human cystathionine gamma-lyase (hCGL) is responsible for methionine-cysteine pathway. hCGL is also the enzyme that can catalyze the formation of hydrogen sulfide together with cystathionine beta synthase in human body. Recently, several mutations in hCGL have been described in patients with cystathioninuria, a rare but poorly understood genetic disease. Cystathioninuria can also be secondarily associated with a wide range of diseases including Diabetes insipidus, Down's syndrome, Neuoroblastoma, Hepatoblastoma, and Celiac disease. To characterize the enzymatic properties of the cgl gene overexpressed in E. coli, we investigated the various conditions for overexpression of pNIC28-Bas4 plasmid which has the T7 promoter under the control of lacO gene. Although the crystal structure was recently reported, the overexpression of hCGL is still important to pursue the studies of reaction mechanism and substrate specificities. Here we report that the optimum conditions such as low culturing temperature, very low aeration during the overexpression, and low concentration of IPTG for the induction are necessary for hCGL overexpression.

키워드

저자

• Kwang-Hwan JHEE [ Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701. ]
• Hae-Jung BYUN [ Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701. ]
• Kyoung-Ran KIM [ Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701. ]
• Seun-Ah YANG [ Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi, 730-701. ]
• In-Seon LEE [ The Center for Traditional Microorganism Resources, Keimyung University, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576