Earticle

초록

영어

Leuconostoc is a major lactic acid bacterium that produces D-lactate in fermented foods. Excessive dietary intake of D-lactate may cause metabolic stress in both infants and patients. Introduction of ldhL gene from Lb. plantarum into Leuconostoc citreum did not significantly shift D-lactate to L-lactate due to low L-LDH activity. To elevate the expression level of ldhL so that significantly alter D-/L-lactate ratio, nucleotides for pyruvate kinase promoter were fused to ldhL and cloned with pLeuCM42 to construct pLC42pkL. Introduction of pLC42pkL in L. mesenteroides subsp. mesenteroides ATCC8293 significantly improved both L-LDH activity and L-lactate productivity during MRS fermentation so that D-/L-lactate ratio was altered significantly. These results indicated that the enhanced L-LDH activity and L-lactate synthesis contributed to the active promoter of native pyruvate kinase and appropriate delivery vector of pLeuCM42. The resulting construct of actively expressing ldhL will further effectively applied in fermented foods. Furthermore, this work provides evidence that expression of other heterologous genes may facilitate the production of useful proteins in these economically important bacteria.

키워드

저자

• Eun-Young SEO [ Dept. of Food Science and Technology, Chungbuk National University ]
• Qing JIN [ Dept. of Food Science and Technology, Chungbuk National University ]
• Yu Jin KIM [ Dept. of Food Science and Technology, Chungbuk National University ]
• Nam Soo HAN [ Dept. of Food Science and Technology, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576