Sangyoung YOON, Eunsoo HONG, Sohyun KIM, Pyungcheon LEE, Yeonwoo RYU
언어
영어(ENG)
URL
https://www.earticle.net/Article/A104760
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원문정보
초록
영어
Aureobasidium pullulans has been known as microorganisms that produce a large amount of β-1,3-1,6-glucan (exopolysaccharides) used for health foods and cosmetics. To achieve production of higher EXPs (exopolysaccharides), we have optimized media for EXPs by using OFAT (one factor at a time) method and stastical analyses (Plackett-Burman design and Box-Behnken design). Basal medium were determinred in Czapek-Dox broth, Ashbya gossypii extract and amonium sulfate instead of sodium nitrate. Our study showed that sodium nitrate played an important role in cell growth and EXPs production. The relative importance of medium components for maximum glucan production was eavlauated by using Plackett-Burman design. It was showed that sucrose, amonium sulfate and A. gossypii extract were significant components for EXPs production. Also, on the basis of Plackett-Burman design analysis, Box-Behnken design was carried out to search for optimum medium of EPSs production. As a result of the box-behnken design and response surface methodology, EXPs production of the optimum medium was two or three times higher than one of the basal medium(5.34 g/L).
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동