Earticle

초록

영어

Transglutaminase (TGase, EC 2.3.2.13) catalyses an acyl tansfer reaction between the primary grade amine and protein or γ-carboxyamide group of peptide bound glutamine residues. TGase is used as a material of many food processing stuffs jelling the protein foods after the formation of cross-links. The microbial TGase gene was cloned from Streptomyces platensis YK-2, which was newly isolated from a forest soil sample collected in Daegu. TGase gene (stgA) was obtained by PCR reaction, Southern blot, and Colony hybridization from Streptomyces platensis YK-2. To construct a high expression vector for stgA, a 1.5 kb fragment including the whole TGase gene was cloned into BamHΙ and XbaI-digested pSET152ET. The pSET152ET vector containing a stgA gene was introduced into S. platensis YK-2 as the host for TGase production by transconjugation using E. coli ET12567 (pUZ8002). Exconjugants were then confirmed by PCR and Southern hybridization. Also, the expression and activity of TGase were assayed by SDS-PAGE and N-terminal protein sequencing, and compared with the strain including only pSET152ET vector.

키워드

저자

• Se-Joung BAE [ Department of Food Science and Biotechnology, Kyungnam University, ]
• Bo-Youn JANG [ Department of Food Science and Biotechnology, Kyungnam University, ]
• Yang-Ho JO [ Department of Food Science and Biotechnology, Kyungnam University, ]
• Sang-In JUNG [ Department of Food Science and Biotechnology, Kyungnam University, ]
• Hyun-Soo KIM [ Department of Microbiology, Keimyung University ]
• Yong-Il HWANG [ Department of Food Science and Biotechnology, Kyungnam University, ]
• Sun-UK CHOI [ Department of Food Science and Biotechnology, Kyungnam University, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576