Earticle

초록

영어

Newcastle disease virus (NDV) has come into the spotlight because of its oncolytic property for cancer therapy and usefulness as a viral vector for vaccine development (1, 2). For the clinical use, the NDV production is performed in cell-based system rather than traditional egg-based system for compliance with GMP/GLP (4). In this study, the rapid detection and quantitation method for the NDV produced in cell-based system was developed. The SYBR Green I-based real-time RT-PCR was designed with conventional cheap RT-PCR kit by targeting F gene of the NDV LaSota strain. The developed method was validated by the contents of specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantitation (LOQ) and robustnesss (4-7). The validation results are satisfied with the predetermined acceptance criteria. The validated method was applied to quantitate the virus samples produced in cell-based production system. And the method was compared with other quantitating methods (3, 7). The comparison results showed that the real-time RT-PCR method would be well adequate to detect and quantitate the whole virus particles containing both infective and uninfective rapidly.

키워드

저자

• Juno JANG [ Dept. of Biotechnology, Korea University, Seoul, 136-701. ]
• Sung-Hwan HONG [ Dept. of Biotechnology, Korea University, Seoul, 136-701. ]
• Ik-Hwan KIM [ Dept. of Biotechnology, Korea University, Seoul, 136-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576