Earticle

초록

영어

In the previous study, we reported that indigo was produced by recombinant E. coli harboring a flavin-containing monooxygenase gene. This recombinant E. coli produced 5.6g/L/day of indigo for 5day on the tryptophan medium (0.2% tryptophan, 1% NaCl, 0.5% yeast extract) through fed-batch cultivation. In this study, investigated the expression of fmo gene in the E. coli DH5α, Bacillus subtilis and Corynebacterium glutamicum to improve the stability of indigo production. The fmo gene(1686bp) was amplified and subcloned into the vectors such as PHY300PLK, pEKEx2, pHCEIIB, pGEX4T-1 and PBSK. The recombinant plasmids produced indigo such as 182 mg/L (B. subtilis/pHY300PLK), 377mg/L (C. glutamicum/pEKEx2), 672.3 mg/L (E. coli DH5α/ pBSK SKII), 400.2 mg/L (E. coli DH5α/pHCE II B) and 638.5 mg/L (E. coli DH5α/pGEX4T-1), respectively.

키워드

저자

• Gui Hwan HAN [ Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]
• Seong Hun IM [ Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]
• Jang Min PARK [ Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]
• Si Wouk KIM [ Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576