Earticle

초록

영어

Over the past 40 years, there was significant increase in research towards the field of molecular electronic to overcome the limitations of silicon based devices. Among several types of molecular devices, molecular memory using biomaterial is a major research theme nowadays. The naturally occurring biomaterials like metalloproteins are nano-sizes and possess inherently charge retention property, which make them to use as a high density molecular device platform. Pseudomonas aeruginosa azurin, the protein of interest in this research, has copper as a key element in the electron transfer mechanisms. This can be used as an electron acceptor in the development of molecular electronic device by mimicking biological mechanism. To achieve their information storage properties on solid state with reproducibility and reliability, the robust fabrication of molecular layers with fine orientation are one of the key processes. We have modified azurin with different number of cysteine residues at its amino acid chain based on site-directed mutagenesis. The resulting recombinant protein, azurin, retained its original redox property in the same manner as native azurin. Recombinant azurin was immobilized on Au substrate by strong affinity between thiol of cysteine and gold. The orientations of recombinant azurin with cystein residues immobilized on the Au substrate were analyzed by fluorescence microscope, scanning tunneling microscope, and surface plasmon resonance. Our data revealed that binding activity of recombinant azurin with two cystein residue on the Au substrate significantly increased in comparison to single residue auzrin. Immobilization of highly oriented recombinant azurin based on cysteine-modification could be useful for the nanoscale fabrication of bioelectronic device. Acknowledgments: This research was supported by the Nano/Bio Science & Technology Program of the Ministry of Education, Science and Technology (MEST), by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (2006-05374), and by Seoul Research and Business Development Program (10816).

키워드

저자

• Sang-Uk KIM [ Interdisciplinary Program of Integrated Biotechnology, Sogang Univ. ]
• Jin-Ho LEE [ Department of Chemical & Biomolecular Engineering, Sogang Univ. ]
• Taek LEE [ Department of Chemical & Biomolecular Engineering, Sogang Univ. ]
• Junhong MIN [ College of Bionanotechnology, Kyungwon Univ. ]
• Jeong-Woo CHOI [ Interdisciplinary Program of Integrated Biotechnology and Department of Chemical & Biomolecular Engineering, Sogang Univ. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576