Earticle

초록

영어

Opium poppy (Papaver somniferum) plant is known as a commercial source of morphinan alkaloids such as thebaine, codeine and morphinee. But callus induced from this plant are regarded to lose their ability to produce morphine and related alkaloids, which were derived from (R)-reticuline and produced in high concentration by the original intact plants, due to lack of enzymes transforming (R)-reticuline and codeine to next metabolites. Therefore, to elucidate the differences between secondary metabolism of plant cells and intact plants of Papaver somniferum and to determine the potential step for targeted metabolic engineering, we compared the expression profiles of gene transcripts and enzymes on the alkaloid biosynthetic pathway in P. somniferm using plant cells and intact plants parts such as leaves, stems, roots and capsules. Gene transcript and protein expression level of salutaridine oxidoreductase in cells was negligible compared to that in intact plants. Through integrated-omics study on secondary metabolic pathway of P. somniferum salutaridine oxidoreductase (SR) could be suggested as a potential target of metabolic engineering for cells whose secondary metabolism would be altered by genetic manipulation to produce morphinan alkaloids in vitro.

키워드

저자

• Hong Soon RHEE [ Department of Chemical Engineering, Advanced Environmental Biotechnology Research Center, POSTECH, Pohang, 790-784. ]
• Seok Young SON [ Department of Chemical Engineering, Advanced Environmental Biotechnology Research Center, POSTECH, Pohang, 790-784. ]
• Jong Moon PARK [ Department of Chemical Engineering, Advanced Environmental Biotechnology Research Center, POSTECH, Pohang, 790-784. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576