Earticle

초록

영어

Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from the plant cell cultures of Taxus chinensis. Crude paclitaxel was dissolved in methanol and the solution was diluted with distilled water to 61.5% (v/v) methanol. The water feeding and mixing method of fractional precipitation were evaluated in terms of the yield, purity and precipitation time of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v) , and storage time ( 7 hr and 14 hr ). The maximum purity (~57.4%) and yield (~81.8%) of paclitaxel were obtained at pH 8 with ammonium phosphate buffer. The yield and purity of paclitaxel were increased by repeated intermittent addition of ammonium phosphate buffer during fractional precipitation. This evaluation of fractional precipitation process serves to minimize solvent usage and the size and complexity of the high performance liquid chromatography operation required for paclitaxel purification.

키워드

저자

• Da-Hye LEE [ Department of Chemical Engineering, Kongju National University, ]
• Seul-Gi KIM [ Department of Chemical Engineering, Kongju National University, ]
• Jin-Hyun KIM [ Department of Chemical Engineering, Kongju National University, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576