Optimization of carbon and nitrogen sources, and initial pH of medium for production of cellulases by Cellulophaga lytica LBH-14 using an orthogonal array method
This experiment is concerned with optimization of culture medium by C. lytica LBH-14. The one-factor-at-a-time method was used to investigate the effect of carbon & nitrogen sources, and initial pH on production of carboxymethylcellulase (CMCase) and cellobiase by C. lytica LBH-14. Base on the result of one-factor-at-a-time experiment, L25-53 was performed to measure combined effects of carbon source & nitrogen source, and initial pH on production of CMCase and cellobiase. Optimal conditions for production of CMCase were 10.0% (w/v) rice bran, 0.75% (w/v) ammonium nitrate, and initial pH 6.00, whereas those of cellobiase were 10.0% rice bran, 0.50% ammonium nitrate, and the initial pH of 7.50. On basis of intuitive analysis and statistical calculations using data from an orthogonal experiment, ammonium nitrate was found to be the most important factor for production of the CMCase by C. lytica LBH-14, whereas the most important factor for production of cellobiase was rice bran.
키워드
C. lytica LBH-14orthogonal experimentCMCase
저자
WA GAO [ Department of Medical Bioscience. Dong-A University. ]
Yi-Joon KIM [ Department of Medical Bioscience. Dong-A University. ]
You-Jung LEE [ Department of Medical Bioscience. Dong-A University. ]
Jin-Woo LEE [ Department of Biotechnology, Dong-A University. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
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