Earticle

초록

영어

Quantification of RNA provides the crucial information in various fields of biology, such as transcriptomics, pathogen detection. The major technology for RNA quantification is real-time PCR, which is known as the most accurate method of nucleic acids quantification. However, application of real-time PCR on RNA quantification is limited by single target per an analytical run because of reduced quantification power in multiplex detection and limitation on fluorescence dye. Here, we report a novel multiplex RNA quantification method using capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) coupled with asymmetric PCR using common primers. There are three major steps which are template modification, multiplex amplification, and simultaneous detection. Firstly, RNA targets are modified for multiplex amplification step. After the first step, all targets have the same end-sequences, and the sequences are the sites on which the common primers hybridize. Second step is multiplex amplification of targets using asymmetric PCR with the common primer pair. Finally, the amplicons are separated and quantified by CE-SSCP analysis.

키워드

저자

• Gi Won SHIN [ I-Bio Program, POSTECH. ]
• Hee Sung HWANG [ I-Bio Program, POSTECH. ]
• Gyoo Yeol JUNG [ I-Bio Program and Dept. of Chemical Engineering, POSTECH. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576