Earticle

초록

영어

Mussel adhesive protein (MAP) fp-151 designed and constructed by our research laboratory has the potential to be used as a cell or tissue bioadhesive at various cell lines in biocompatible aspect. To improve the cell-adhesion and proliferation properties of fp151, we designed an extracellular matrix (ECM) mimics based on mussel adhesive protein fused with biofunctional peptides such as RGD, laminin, type Ⅳ collagen, growth factor called substance P, which is fused at Cterminus of fp151. The ability of adhesion and proliferation properties is tested in several cell lines by direct cell count (DCC) method and MTT assay. Our ECM mimics showed better or similar adhesion and proliferation properties compared with other commercially produced cell-adhesion materials such as poly-L-lysine (PLL) and the naturally extracted MAP mixture Cell-Tak in MC3T3-E1, ATDC5 and 3T3-L1 cells. These data showed that these mimics can be successfully used in cell culture and tissue engineering and extended to other tissuespecific cell recognition motifs to allow attachment of target cells to artificial ECM surfaces.

키워드

저자

• Bong-Hyuk CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. ]
• Yoo Seong CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. ]
• Young Hoon SONG [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. ]
• Hyung Joon CHA [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576