The Bioprocessing Technology Institute (BTI) was created in 2003 to be the strategic global partner in bioprocess research, positioning Singapore as the biomedical and biomanufacturing hub in the Asia-Pacific region. BTI has invested significant resources to establish capabilities in Immunology, Expression Engineering, Animal Cell Technology, Microbial Cells, Stem Cells, Downstream Processing, Analytics and ‘-Omics’ Technologies. As an example of this investment in R&D, I will showcase our capabilities in Stem Cell Bioprocessing. An important challenge that is faced in stem cell bioprocessing is how to exploit the unique replicative and differentiation properties of human embryonic stem cells (hESC) which make them such excellent candidates for allogeneic cell therapy. At present, there is no robust method for producing gigantic quantities of hESC that will be required for human clinical trials. To meet that need, we have developed a method for 3 dimensional (3D) microcarrier culture of hESC which allows facile expansion of these cells to 2 to 4 fold higher densities than conventional 2 dimensional (2D) colony cultures. hESC can be cultured continuously on microcarriers for 6 months while retaining their normal karyotype and their ability for differentiation. We have demonstrated this capability both in conditioned media and 2 serum free media and shown the ability to grow hESC in spinner flask suspension cultures. Long term culture of hESC is possible with rod shaped as well as spherical microcarriers, with matrigel, hyaluronic acid and other extracellular matrix coatings on these microcarriers. Human iPS cells have also been expanded on microcarriers and the platform is versatile enough to differentiate stem cells to beating cardiomyocytes which will be useful in cell therapy applications for the heart.
저자
Steve Oh [ Stem Cell Group, Bioprocessing Technology Institute (BTI), Agency for Science Technology and Research, (A*STAR), SINGAPORE ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동