Earticle

초록

영어

Truffles are hypogeous ascomycetes and ectomycorrhizal fungi with unique organoleptic properties, which is one of the most valuable gourmet mushrooms on the market. Recently, many ingredients including androstenol, ceramides and Tuber polysaccharides have been isolated from the fruiting-bodies and fermentation mycelia of truffle and their biological activities
have been identified. The androstenol could modulate the female catamenia and heighten female sexual arousal. Ceramides have bioactivities such as moisture-retaining, apoptosis inducing, antitumor and immunostimulatory. Tuber polysaccharides also have immunomodulating and antitumor activity. Nowadays, truffles are mostly obtained from the nature and semiartificial
simulation cultivated. However, the former is scarce; the latter is time-consuming which usually takes 7-9 years to harvest fruiting-body. Truffle fermentation to produce mycelia, extracellular polysaccharides (EPS), and intracellular polysaccharides (IPS) was developed for the first time in our lab. Significances of carbon source, nitrogen source and metal ion were studied in details. Sucrose was the suitable carbon source and its optimal initial concentration was 80 g/L. Yeast extract and peptone were beneficial for truffle fermentation and their optimal initial concentration was 25 g/L and 30 g/L, respectively. A model constructed by Box-Behnken design was applied to study the synergic effect of glucose, yeast extract and peptone, and their optimal initial concentration was 60, 30 and 10 g/L, the maximal mycelia biomass, EPS production and IPS
production was 24.84 g/L, 1.62 g/L and 1.51 g/L, respectively. Mg2+ and K+ significantly affected the EPS biosynthesis. EPS production reached its peak value of 5.86 g/L under the optimal combination of 30 mM Mg2+ and 5 mM K+. Base on the results obtained above, a fed-batch fermentation process was developed. Each 1.67 g/L of peptone and 8.33 g/L of yeast extract
were added on day 3, 6, and 9, respectively, and sucrose was fed to maintain its concentration around 35-5 g/L when its residual level decreased to 10-5 g/L. Then, the maximal biomass, the production EPS and IPS reached 53.72±2.57 g DW/L, 7.09±0.62 and 4.43±0.21 g/L, which was enhanced by 55.8%, 222.3% and 103.2%, compared with the batch culture, respectively. Not
only the cell density but also the production of EPS and IPS were the highest ever reported in truffle fermentation. And the biomass was also the highest as ever reported in mushroom fermentation. Submerged culture of truffle is viewed as a promising alternative for efficient production of valuable metabolites and has received increasing attention around the world.

저자

• Ya-Jie Tang [ Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China ]
• Rui-sang Liu [ Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China ]
• Guan Wang [ Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China ]
• Ling-Li Zhu [ Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China ]
• Qiao-Ning Liu [ Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576