Earticle

초록

영어

Lipid bilayers and their biomimetic analogs are used in a variety of scientific applications and have also been explored as a platform for highly sensitive and rapid single molecule sensing. Furthermore, ion channels incorporated into such biomimetic membrane have a number of
applications and potential uses including drug screening, drug discovery, and electrophysiological studies. However, these uses are inhibited by the fragility and short lifetime of the membrane supporting the protein and lipid bilayer technologies have historically been the purview of experts, precluding its use as a high-throughput application. In order to address
those shortcomings, we are developing an easy-to-use, robust, automatable, and inexpensive technological platform for membrane formation and channel protein measurement. Technologies to realize such platform includes membrane stabilization by hydrogel encapsulation/conjugation,
freezing of membrane precursors, and automated high throughput membrane formation. With these techniques, membranes we have created could withstand severe perturbation, support long-term continuous measurements, and were shippable and storable. Additionally, these
techniques are compatible with high throughput robotic systems for drug screening. We will present our recent progress in developing a platform integrated with the multiplexed electrical measurement of ion channels in a 96 well format, completely removing the operator from the membrane formation and its measurement process, which will result in a reliable highthroughput
drug screening platform

키워드

저자

• Tae-Joon JEON [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576