Earticle

초록

영어

Riboregulatory genetic control has been attracting increasing attention as recent research reveals that noncoding RNA elements and ligandbinding regulatory mRNAs play pivotal roles in control over celluar gene expression. We are interested in constructing synthetic biological circuits for biomedical applications by exploiting the riboregulatory elements. Here, we present engineering of biological circuits by combining a theophylline-mediated riboswitch and a TetR-regulatory
transcriptional control system, so the resulting biological circuits controllable at both transcriptional and translational levels can be regulated by theophylline. The synthetic gene circuit consists of two components, a sensor plasmid constructed by incorporating a theophylline
aptamer sequence into a coding region of tetR gene and a reporter plasmid prepared by placing an egfp gene under the control of TetR repressor. We constructed six combinations of the two plasmids of different replication origins, so the resulting plasmid combinations show a varying number of plasmid copy number. We found that one combination among six showed the best circuit behavior, indicating that connecting the riboregulation and the transcriptional control
requires subtle balancing of the two regulatory elements.

키워드

저자

• Jong-Shik SHIN [ Department of Biotechnology, Yonsei University. ]
• Jeongtae SON [ Department of Biotechnology, Yonsei University ]
• Joonjung JO [ Department of Biotechnology, Yonsei University. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576