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Bioneer Synthetic Biotechnology Symposium
Autoinduction Expression System in Synthetic Biology Era
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- 한국생물공학회 학술대회 바로가기
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- 통권
- 2009 춘계학술대회 및 국제심포지움 (2009.04)바로가기
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- 페이지
- pp.60-60
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A104602
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Ability to write and synthesize DNA is fundamentally changing the way of research and approach in biotechnology research. However purposeful modification of metabolic pathways, metabolic engineering, is still limited by tuned expression of genes. In this presentation, we will review basic needs of expression systems in the era of synthetic biology and report our work on autoinduction expression systems. Firstly, we developed autoinduction system by using acs promoter. Acetyl-CoA synthetase(Acs) is responsible for the consumption of acetate accumulated during growth phase. Repressed in the presence of glucose and highly expressed at the stationary phase or at the limited growth phase, it suits many aspects of the ideal
expression system in synthetic biology applications. pACE plasmid vectors derived from a pUC containing acs promoter was constructed and tested for the expression of LacZ and GFPuv as reporter proteins. Expression of the reporter proteins was tightly controlled with the carbon source: When glucose was used as a sole carbon source, the expression of LacZ was completely repressed during exponential growth phase and induced only at the stationary phase. When glycerol or organic acids were used, the LacZ was constitutively expressed. The uniform expression of GFPuv in each cell at the stationary phase was confirmed by flow cytometry analysis. This promoter was further evolved to display diverse expression profiles. This acs-based expression would provide a novel autoinduction system, enriching various expression systems available currently in Escherichia coli. A similar expression system is under
development in Bacillus subtilis, which will be discussed in detail during the presentation. (Supported by SynBio Cluster Program, MOKE, Korea)
저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- 한국생물공학회 학술대회
- 간기
- 반년간
- 수록기간
- 1985~2013
- 십진분류
- KDC 476 DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움
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