Seoku Kang, Younghoon Kim, Inshick Cho, Kwang-Hyun Kim, Sejong Oh, Ja-Heon Kang, Ilbyung Chung
언어
영어(ENG)
URL
https://www.earticle.net/Article/A103111
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원문정보
초록
영어
Two-dimensional gel electrophoresis (2-DE) was employed to assess the thermo-tolerance characteristics ofBifidobacterium infantis ATCC 27920 to mild heat adaptation. When exposed to various heat levels, pH, and hydrogen peroxide(H2O2) stress conditions, B. infantis ATCC 27920 exhibited high level of stress resistance. Under mild-heat treatment (46oC), nosignificant change in viability level was observed after 2 hr. Interestingly, improved viability was observed in mild-heat adapted(46oC for 1 hr) cultures exposed to 55oC, in comparison to control experiments. Viability was not affected by pH, bile, and H2O2stress conditions. 2-DE analysis revealed those mild-heat adaptation up-regulated 4 proteins and down-regulated 3 proteins.Among these protein spots, isopropylmalate dehydratase (leuD), glycosyltransferase (glgA), and ribosomal protein L5 (rplE) wereidentified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS).
목차
Abstract Introduction Materials and Methods Results and Discussion References
키워드
Bifidobacterium infantsthermotolerancemild heat stress2-dimensional gel electrophoresis
저자
Seoku Kang [ Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea ]
Younghoon Kim [ Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea ]
Inshick Cho [ Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea ]
Ja-Heon Kang [ Department of Ophthalmology, East-West Neo Medical Center, Kyung Hee University, College of Medicine, Seoul 134-727, Korea ]
Ilbyung Chung [ Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Gyeonggi 441-706, Korea ]
Kwang-Hyun Kim [ Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea ]
Sejong Oh [ Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea ]
Corresponding author