Earticle

초록

영어

This study was conducted to examine the changes in the molecular structure and physiological activities of silkfibroin by gamma irradiation. The results of gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gelelectrophoresis showed that the molecular weight of fibroin was increased depending upon the irradiation dose. Secondarystructure of fibroin determined by using circular dichroism revealed that the ratio of α-helix was increased up to 10 kGy andthen decreased depending upon the irradiation dose. Whereas, the ratio of β-sheet, β-turn, and random coil were decreased andthen increased with an alteration in the α-helix secondary conformation. The 2.2-diphenyl-1-picryl-hydrazil (DPPH) radicalscavenging activity of fibroin was increased by gamma irradiation at 5 kGy, but was decreased above 10 kGy depending uponthe irradiation dose. Also, the inhibition activities of tyrosinase and melanin synthesis of fibroin were increased by gammairradiation. These results indicated that gamma irradiation could be used as an efficient method to make fibroin more suitablefor the development of functional foods and cosmetics.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

저자

• Nak-Yun Sung [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Eui-Baek Byun [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Sun-Kyu Kwon [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Jae-Hun Kim [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Beom-Seok Song [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Jong-il Choi [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Jin-Kyu Kim [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Yohan Yoon [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Myung-Woo Byun [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ]
• Mee-Ree Kim [ Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea ]
• Hong-Sun Yook [ Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea ]
• Ju-Woon Lee [ eam for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664