Earticle

초록

영어

Celandine (Chelidonium majus, family Papaveraceae) is an herb used extensively in traditional Korean medicine.To investigate its antioxidant and antiproliferative activities, the methanolic extract of celandine was introduced. Theantioxidant properties of the extract were tested using various in vitro systems, including hydroxyl radical scavenging assay,DNA damage protection assay, 1,1-diphenyll-2-2-pricylhydrazyl (DPPH) free radical scavenging activity, metal chelatingactivity, and reducing power assay. The extract exhibited stronger antioxidant activity (IC50=7.92 µg/mL) against hydroxylradicals in the Fenton system than butylated hydroxyanisole (IC50=51.46 µg/mL) and α-tocopherol (IC50=67.48µg/mL).Likewise, damage to the plasmid pBR 322 induced by hydroxyl radicals was found to be protected by the extract at aconcentration of 400µg/mL. Cellular proliferation and the induction of apoptosis were also examined by a cellularproliferation assay, flow cytometry, and mRNA expression analysis. Taken together, the extract significantly inhibited thegrowth of HT-29 cells in a concentration- and time-dependent manner, and gradually increased both the proportion ofapoptotic cells and the expression of caspase-3. Overall, our research suggests that celandine possesses antioxidant andantiproliferative properties.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

저자

• Weicheng Hu [ School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 200-701, Korea ]
• Myeong-Hyeon Wang [ School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 200-701, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664