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Rapid Detection of Salmonella enteritidis in Pork Samples with Impedimetric Biosensor: Effect of Electrode Spacing on Sensitivity

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 1 (2009.02)바로가기
  • 페이지
    pp.89-94
  • 저자
    Giyoung Kim, Ji Hea Moon, Ae Son Om, Bung-Kwon Hahm, Mark Morgan, Arun Bhunia
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A103083

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원문정보

초록

영어
Frequent outbreaks of foodborne illness have been increasing the awareness of food safety. Conventional methodsfor pathogen detection and identification are labor-intensive and take days to complete. Some immunological, rapid assays aredeveloped, but these assays still require prolonged enrichment steps. Recently developed biosensors have shown potential forthe rapid detection of foodborne pathogens. In this study, an impedimetric biosensor was developed for rapid detection ofSalmonella entritidis in food sample. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by usinga semiconductor fabrication process. Anti-Salmonella antibodies were immobilized based on neutravidin-biotin binding on thesurface of the IME to form an active sensing layer. To evaluate the effect of electrode gap on sensitivity of the sensor, 3 typesof sensors with different electrode gap sizes (2, 5, and 10µm) were fabricated and tested. The impedimetric biosensor coulddetect 103 CFU/mL of Salmonella in pork meat extract with an incubation time of 5 min. This method may provide a simple,rapid, and sensitive method to detect foodborne pathogens.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

biosensor Salmonella interdigitated microelectrode impedimetric immunosensor

저자

  • Giyoung Kim [ Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi 441-100, Korea ] Corresponding author
  • Ji Hea Moon [ Deptartment of Food and Nutrition, Hanyang University, Seoul 133-791, Korea ]
  • Bung-Kwon Hahm [ Department of Food Science, Purdue University, West Lafayette, IN 47907, USA ]
  • Mark Morgan [ Department of Food Science, Purdue University, West Lafayette, IN 47907, USA ]
  • Arun Bhunia [ Department of Food Science, Purdue University, West Lafayette, IN 47907, USA ]
  • Ae Son Om [ Deptartment of Food and Nutrition, Hanyang University, Seoul 133-791, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 18 Number 1

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