Earticle

초록

영어

B-Glucosidaes from Cellvibrio gilvus(CG) was successfully overproduced in soluble form in E. coli with the coexpression of GroEL/ES/. Without the GroEL/ES protein, the B-glucosidase overexpressed in E. coli constituted a huge amount(80%) of total cellular protein, but was localized in the insoluble fraction, and little activity was detected in the soluble fraction. Coexpression of the E. coli GroEL/ES had a drastic impact on the proper folding of the B-glucosidase; 20% of the overexpressed enzyme was recovered in the soluble fraction in active form. Similar effects of GroEL/ES were also observed on the overexpressed -glucosidase from Agrobacterium tumefaciens(AT). And pET28(a)-RGRAR, partially deleted mutant lacking 5-amino acid residues at carboxy teminus also could be folded into an active form when expressed with the molecular chaperonin GroEL/ES, and its activity was higher than that of the without GroEL/ES system, In addition, the synergistic effect of GroEL/ES and the low induction temperature were important factors for solubilization of the inclusion body from overproduced -glucosidases.

목차

서론
 재료 및 방법
  Bacterial strain and vectors
  배지
  GroEL/ES system
  균체의 배양
  SDS-PAGE
 결과 및 고찰
  균주의 성장
  E. coli 내에서의 B-glucosidase의 과잉 생산
  단백질의 과잉생산 및 chaperonin에 의한 가용화
  Chaperonin에 의한 B-glucosidase의 총 활성의 향상
  저온 배양에 따른 B -glucosidase의 가용성 향상
  Coexpression과 저온 배양의 synergic effect
  Deleted mutant의 chaperonin에 의한 총 활성의 향상
 결론
 참고문헌

키워드

저자

• 김종덕 [ Kim, Jong-Deok | 여수대학교 생물공학과 ] Corresponding Author
• Sachiko Machida [ 일본 농림수산청 식품총합연구소 ]
• Kiyoshi Hayashi [ 일본 농림수산청 식품총합연구소 ]
• 하순덕 [ Ha, Sun-Deok | 부경대학교 생물공학과 ]
• 공재열 [ Gong, Jae-Yeol | 부경대학교 생물공학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576