Earticle

초록

영어

Studies were made to investigate effects of the scale-up of stirred tank bioreactors on cell growth and alkaloids production for suspension cultures of Eschscholtzia californica. In the 1.5 L STR, cell lysis was observed at 110 rpm or higher agitation speed. The agitation speed of 30 L STR was 43.7 rpm to maintain the same shear stress developed in 1.5 L STR of 100 rpm. As a result of scale-up from 1.5 L to 30 L STR, the specific growth rate was decreased from 0.12 to 0.07 day-1. The alkaloids productivity was also decreased from 0.24 to 0.14 mg/L-day. Changes of mixing performance and oxygen transfer were studied to explain the decrease of cell growth and alkaloids production. Decreased oxygen transfer rate coefficient(KLa) and increased mixing time by the scale-up was observed at various aeration rates.

목차

서론
 재료 및 방법
  세포배양 및 배지
  세포생장 측정
  알칼로이드 분석
  당 분석
  세포 생존율(cell viability) 측정
  산소전달계수(oxygen transfer rate coefficient , KLa) 및 혼합시간(mixing time, tm) 측정
  탱크 교반형 생물반응기의 운전
 결과 및 고찰
  전단응력의 영향 및 scale-up 기준
  Scale-up이 세포생장 및 알칼로이드 생산에 미치는 영향
  Scale-up에 따른 산소전달속도 및 교반효율의 변화
 요약
 감사
 참고문헌

키워드

저자

• 유병삼 [ Byoung Sam Yoo | 아주대학교 공과대학 화학·생물공학부 ]
• 변상요 [ Sang Yo Byun | 아주대학교 공과대학 화학·생물공학부 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576