Earticle

초록

영어

Hantaviruses are rodent hosts-borne viruses belonging to the family Bunyaviridae, and are etiologic agents for two acute diseases, i.e., Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome (HPS). There have been a lot of reports on prophylactic vaccines and diagnostics for the diseases, but most of viral antigens have been prepared by eukaryotic cell culture. Nucleocapsid proteins of Hantaviruses are known as the major viral antigens. Thereby, we prepared nucleocapsid genes of Hantaan virus and Seoul virus by RT-PCR and cloned into plasmid vectors, pET-3a and pKK223-3. Both genes were expressed in Escherichia coli with higher expression level of Seoul viral nucleocapsid protein compared to that of Hantaan in pET-3a. Hantaan viral gene was expressed much higher level in plasmid pET-3a that in pKK223-3. About 30% of expressed nucleocapsid protein was soluble and the rest was remained in insoluble fraction.

목차

서론
 재료 및 방법
  박테리아, 바이러스, 플라스미드, nucleotide oligomers
  배지, 효소 및 기타 시약
  유전자 조작, SDS-PAGE 및 western blot
 결과 및 고찰
  뉴클레모캡시드 유전자 (nucleccapsid gene)의 합성
  재조합 플라스미드의 제작
  뉴클레오캡시드 단백질의 발현 및 분석
 요약
 참고문헌

키워드

저자

• 노갑수 [ Kap Soo Noh | 제일제당 종합연구소 ] Corresponding Author
• 하석훈 [ Suk Hoon Ha | 제일제당 종합연구소 ]
• 김종완 [ Jong Wan Kim | 제일제당 종합연구소 ]
• 홍선표 [ Sun Pyo Hong | 제일제당 종합연구소 ]
• 김형배 [ Hyong Bai Kim | 고려대학교 생명공학원 ]
• 최차용 [ Cha Yong Choi | 서울대학교 공업화학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576