Earticle

초록

영어

In the production of a low cost bacterial insecticide, it is important to produce a high spore concentration using low price substrates. Experiments were carried out to investigate the effects of the addition of mineral salts and glucose, and of dissolved oxygen concentration on the cell growth and spore formation of Bacillus thuringiensis var aizawai using a cheap wheat and soybean meal in the batch culture. The maximum viable cell number was 1.2109 CFU/mL at 12 hr culture and spore yield was 54.2% at 74 hr culture using an industrial medium containing 20 g/L wheat meal and 30 g/L soybean meal under 1.0 vvm aeration and 200 rpm agitation. The cell growth and the spore formation were not enhanced by the addition of mineral salts in industrial medium, whereas th addition of 10g/L glucose decreased the cell growth and spore formation. We could obtain a maximum viable cell number of 2.2109 CFU/mL and spore number of 1.9109 CFU/mL at the dissolved oxygen concentration of 60% of saturation. The spore concentration was enhanced approximately by 2 times as compared to the dissolved oxygen concentration of 50%. In the bench-scale culture, the maximum viable cell and spore number were 2.5109 CFU/mL, and 2.2109 CFU/mL, respectively under 1.0 vvm aeration and 400 rpm agitation. The spore yield was 88% based on the maximum viable cell number. As a result, it was confirmed that the production of high spore concentration could be obtained by a bench-scale culture using an industrial medium.

목차

서론
 재료 및 방법
  균주 및 배지조성
  배양조건
  세포 및 포자농도의 결정
 결과 및 고찰
  산업용 배지에 의한 B. thuringiensis의 배양
  무기염과 glucose 첨가에 의한 영향
  용존산소량의 영향
  Bench-scale 의 생산
 요약
 감사의 글
 참고문헌

키워드

저자

• 최성호 [ Sung Ho Choi | 서울대학교 농업생명과학대학 농업생물신소재 연구센터 ]
• 강석권 [ Seok Kwon Kang | 서울대학교 농업생명과학대학 농업생물신소재 연구센터 ]
• 유연우 [ Yeon Woo Ryu | 아주대학교 공과대학 화학·생물공학부 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576