Earticle

초록

영어

Biopolymer from alkaline-tolerant Bacillus so. was purified, and its physico-chemical and structural properties were investigated. Crude biopolymer, precipitated by acetone from culture broth was fractionated into two fractions by gel chromatography on Sephadex G-200. Among two fractions, one fraction(PS I), which an acidic biopolymer precipitated by the CPC(cetylpyridinium chloride) treatment was studied further. PS I fraction had carboxyl groups and was positive at color reaction of sugar. PS I fraction also showed UV absorbance at 190-225nm. The purified acidic biopolymer was composed of 4% glucose, 8% glucosamine and 88% glutamic acid. Sugar components of the purified acidic biopolymer seemed to be linked to PGA(polyglutamic acid) which existed in the from of r-peptide bond. By the results of Smith degradation of sugar components, glucose and glucosamine was bound by 1,3 glocosidic linkage. Therefore, this biopolymer was a glycopeptide, oligosaccaride r-PGA. We concluded that the equivalent weight and the molecular weight of this biopolymer were estimated as about 171 and 5x105 dalton, respectively.

목차

서론
 재료 및 방법
  균주 및 배양
  생물 고분자의 분리 및 정제
  염계 염농도(critical salt concentration)의 결정
  생물 고분자의 이화학적 분석
  구성 성분의 분석
  당의 산 가수분해 양상
  Smith 분해에 의한 당결합 양상의 결정
  중회 당량(neutralization equivalent)의 결정
  분자량의 측정
 결과 및 고찰
  생물고분자의 분획 및 정제
  구성 성분
  UV 및 IR 흡수 특성
  당결합 양상
 요약
 참고문헌

키워드

저자

• 이신영 [ Shin -Young Lee | 강원대학교 환경·생물공학부 ] Corresponding Author
• 원숙 [ Suk Won | 강원대학교 환경·생물공학부 ]
• 강태수 [ Tae-Su Kang | 강원도 농촌진흥원 ]
• 이명열 [ Myong-Yurl ee | 충주산업대학교 식품공학과 ]
• 류인덕 [ In-Deok Lew | 충주산업대학교 식품공학과 ]
• 김진영 [ Jin-Young Kim | (주) 김정문 알로에 연구소 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576