Earticle

초록

영어

Stabilization of antibody, which is specific to Salmonella typhimurium antigens, present in dry states on membranes was accomplished, and its shelf-life, i.e., duration for maintaining minimum 90% of the initial activity, under optimal conditions was determined. To prepare two major components of an immuno-strip, the antibody was not only immobilized on nitrocellulose membrane surfaces but also placed within the pores of glass fiber membrane after conjugating it with old colloids as signal generator. Among potential stabilizers of the immuno-components, a disaccharide, trehalose, showed a significant protection effect of immunoglobulin structure from thermal energy. Optimal concentrations of trehalose for the respective component were significantly different (8-fold higher for the antibody-gold conjugate than for the immobilized antibody), which probably resulted from distinct densities and configurations of antibody present on the membranes. An additional requirement for the gold conjugate was freeze-drying of this substance such that the conjugate can be readily resolubilized upon contact with aqueous medium. By using the components prepared under optimal conditions, immuno-strips were constructed and exposed to thermal energy. Signals with less than 10% decrease in the intensity were maintained for approximately 21 days at 60℃. Compared to previous reports, this result represented a 2-year shelf-life at room temperature. it was, however, two times longer if determined from thermal acceleration tests based on the theory of inactivation rate of protein. Such discrepancy between the two estimates could be mainly attributed to errors in accurately controlling temperatures and also to changes in the physical properties of membranes due to a high thermal energy.

목차

서론
 재료 및 방법
  재료
  항체-colloidal gold 중합체 합성
  안정제의 선택
  포획항체의 안정화
  탐지항체-gold 중합체의 안정화
  분석시스템의 열 안정성 시험
  Shelf-life 산출
 결과 및 고찰
  단백질 안정화
  분석시스템의 열 안정성
  Shelf-life 결정
 요약
 감사
 참고문헌

키워드

저자

• 이창우 [ Chang-Woo Lee | 고려대학교 생명공학원 ]
• 조정환 [ Joung-Hwan Cho | 고려대학교 생명공학원 ]
• 육순학 [ Soon-Hack Yook | 두산인재기술개발원 과제개발분야 ]
• 권오협 [ Oh-Hyep Kwon | 두산인재기술개발원 과제개발분야 ]
• 박영남 [ Yeong-Nam Park | 영동제약 연구개발센터 ]
• 박세환 [ Se-Hwan park | 고려대학교 생명공학원 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576