Earticle

초록

영어

The possibility of application of membrane type immobilized adsorbent to the fed-batch or perfusion culture system with anchorage-independent cells as well as batch system was investigated. The improvement in cell density and cell viability due to the combination of immobilized adsorbent with each culture system was evaluated for the investigation, and the optimum culture system employing immobilized adsorbent system was suggested based on the results. It was observed that the system with immobilized adsorbent showed better cell growth and cell viability than that without immobilized adsorbent in every operation system of batch, fed-batch, and perfusion. In case of batch system, 200% improvement of maximum cell density was observed in the system where ammonium chloride was added on purpose. And 50% improvement of maximum cell density was observed in the fed-batch system where ammonium ion accumulates significantly, while small increase in maximum cell density was observed in the perfusion system where dilution of waste byproducts exists. Especially, the fed-batch system showed the most significant improvement on cell growth because both compensation of nutrient and removal of ammonium ion occurred simultaneously in the system. Therefore a combined system of immobilized adsorbent and fed-batch operation could be suggested as an optimum system with in situ removal of ammonium ion.

목차

서론
 재료 및 방법 
  모델 세포주
  배지 및 세포배양 조건
  Membrane type 고정화 흡착제
  고정화 흡착제의 여러 배양 시스템의 응용
  세포농도 및 생존율, 암모늄 이온 농도 분석
 결과 및 고찰
  고정화 흡착제의 회분식 CHO 세포 배양에의 응용
  제목없음
  고정화 흡착제의 유가식 배양 공정(Fed-batch culture System)에의 응용
  고정화 흡착제의 Perfusion 배양 공정에의 응용
 결론
 요약
 감사
 참고문헌

키워드

저자

• 박병곤 [ B. G. Park | 강원대학교 식품생명공학부 ]
• 이해익 [ H. I. Rhee | 강원대학교 식품생명공학부 ]
• 전계택 [ G. T. Chun | 강원대학교 생명공학부 ]
• 김익환 [ I. H. Kim | 고려대학교 생명공학원 ]
• 정연호 [ Y. H. Jeong | 강원대학교 식품생명공학부 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576