Earticle

초록

영어

Bacillus subtilis DT134 was resistant to 50-fold higher concentration of cadmium ions (Cd2+) than cadmium-sensitive B. subtilis BD224 in Luria Broth (LB) medium. Minimal inhibition concentration test in LB agar plates also showed similar results. The elevated cadmium resistance of B. subtilis DT134 strongly suggested a possible existence of cadmium resistance gene in it. Southern blot with Staphylococcus aureus cadA gene fragment (757 bp NlaIV-XmnI cadA DNA fragment) as probe was carried out to test the existence and similarity of the gene. In high stringency condition, there was no detectable signal, but in low stringency, a strong signal specific to the cadA probe could be detected. These results strongly suggested that there was some similarity between total DNA of B. subtilis DT134 and S. aureus pl258 in terms of cadmium resistance gene and the resistance mechanism might be an efflux mechanism. The subsequent efflux experiment showed that the cadmium resistance mechanism of B. subtilis DT134 was also due to the efflux of cadmium.

목차

Introduction
 Materials and Methods
  Baclerial Strains and Plasmids
  Materials
  DNA Fragment Punlication
  Growth Inhibition
  Mininal Inhibitory Concentrations
  Construction of Plasmids pKPY26
  Preparation of Biotin Labeled Probe DNA
  Soouthern Blot Analysis
  Efflux Assay
 Results and Discussion
  Growth Inhibition
  Minimal Inhibitory Concentration
  Construction of Plasmid pKPY26
  Southern Blot Hybridization
  Efflux Assay
 요약
 Acknowledgement
 References

키워드

저자

• 윤경표 [ Kyung Pyo Yoon | 계명대학교 자연과학대학 미생물학과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576