Earticle

초록

영어

The effects of various step-fortifications of the initial medium with amino acids, glucose, and vitamines on the growth and metabolism of a hybridoma cell line in batch cultures were quantified. Comparisons between the metabolic rates of the various cultivations were made for the exponential growth phase. Fortification of the basal medium resulted in higher cell densities through a prolonged growth phase, but the maximum specific growth rate was not affected. The uptake rate of glutamine increased with the addition of amino acids but did not change upon the addition of glucose or vitamines. The specific glucose consumption decreased slightly with the addition of amino acids but increased production of lactate and {{{{ { NH}`_{4 } ^{ +} }}}}. A reciprocal relationship between the yields of {{{{ { NH}`_{4 } ^{+ } }}}} and lactate indicated a joint regulation of glycolysis and glutaminolysis.

목차

서론
 재료 및 실험방법
  실험방법
  세포의 비성장속도, 기질의 비소비속도, 노폐물의 비생성속도의 계산
 결과 및 고찰
  배지강화에 따른 세포성장의 변화
  배지강화에 따른 영양원 소비의 변화
  배지강화에 따른 노폐물 생성의 변화
 요약
 감사
 참고문헌

키워드

저자

• 이동섭 [ Dongsup Lee | 한양대학교 화학공학과 ]
• 박홍우 [ Hongwoo Park | 한양대학교 화학공학과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576