Earticle

초록

영어

Production of inulo-oligosaccharides from inulin was carried out with the maximum yield of 94% using a purified endoinulinase from Aspergillus ficcum. The optimum reaction temperature and pH were 55-60 and pH 5.5-6.0, respectively. The Michaelis constant and maximum reaction velocity of the endoinuinase for inulin were 13.27 g/L and 0.13 g/Lmin at 55, pH 5.5, respectively. Inulin source had no significant effect on oligosaccharide yield and product composition, although initial production rate differed according to inulin origins. The reaction pH was a critical factor in inulo-oligosaccharide production because considerable free monosaccharides were released, decreasing oligosaccharide yield at low pH conditions. An empirical relationship describing the reaction performance was developed from kinetic data: the time to reach maximum oligosaccharide yield (tw) as a function of initial concentration of inulin (lo) and enzyme (Eo); i.e., log tM = -1.025 log Eo - 0.011 l0 + 2.655.

목차

서론
 재료 및 방법
  실험재료
  정제효소
  효소활성 측정
  효소반응
  분석
 결과 및 고찰
  정제효소의 반응특성
  기질형태에 따른 이눌로올리고당의 생산특성
  반응 pH의 영향
  초기 이눌린농도 및 효소량의 영향
 요약
 감사
 참고문헌

키워드

저자

• 윤호범 [ Ho Bum Yoon | 부산대학교 화학공학과 ]
• 김동현 [ Dong Hyun Kim | 대구대학교 생물공학과 ]
• 윤종원 [ Jong Won Yun | 대구대학교 생물공학과 ]
• 김병우 [ Byung Woo Kim | 동의대학교 미생물학과 ]
• 송승구 [ Seung Koo Song | 부산대학교 화학공학과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576