Earticle

초록

영어

For mass production of polyhydroxyalkanoates (PHA), high cell density cultures of Alcaligenes eutrophus by fed-batch culture under phosphate-limitation condition has been investigated. PHA accumulation by the regulation by the regulation of initial phosphate concentration could be automatically induced, and high density cell culture above 200 g/L also could be successfully produced. The production of Poly-B-hydroxybutyrate (PHB) and dry cell weight increased with increasing the initial phosphate concentration. When the initial concentrations of phosphate were in the ranges of 1.5~4.5 g-PO4/L, PHB and dry cell weight obtained were 83~266 g/L and 61~216 g/L, respectively, and PHB productivity was in the ranges of 1.35~3.10 g/L.h. When a mixture of glucose and propionic acid is used as carbon sources, poly(3-hydroxybutyrate-co-poly-3-hydroxyvalerate), P(3HB-co-3HV), could be also successfully produced under phosphate limitation condition. When the mole ratio of propionic acid to glucose in the feeding solution is 0.22, a final dry cell weight of 150 g/L and a P(3-HB-co-3HV) of 90 g/L were produced. Morphological changes and size distribution of PHB granules synthesized in A. eutrophus under phosphate-limitation condition are determined by TEM during the course of fed-batch. Mean granule diameters of PHB produced are in the range of 0.36~0.39 m, and mean cell size was elongated from 0.54~0.59 um 1.3~1.5 m to 0.83~0.89 m 2.0~2.3 m. Phosphate concentration in media did not affect size distribution of PHB granule and cell.

목차

ABSTRACT
 서론
 재료 및 방법
  균주 및 배지
  유가식 배양법에 의한 PHB의 생산
  P(3HB-co-3HV)의 생산
  분석방법
 결과 및 고찰
  인산염 제한하에서의 PHB의 생산
  인산염 제한하에서의 P(3HB-co-3HV)의 생산
  세포 크기의 변화
  PHB 입자크기의 변화
 결론
 감사
 참고문헌

키워드

저자

• 류희욱 [ Hee Wook Ryu | 숭실대학교 환경·화학공학과 ] Corresponding Author
• 조경숙 [ Kyung Suk Cho | 이화여자대학교 환경공학과 ]
• 장용근 [ Yong Keun Chang | 한국과학기술원 화학공학과 및 생물공정연구센터 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576