Earticle

초록

영어

The hairy roots of Crotalaria sessiliflora were induced from the tissue segments infected with Agrobacterium rhizogenes ATCC 15834. The induced hairy roots were subjected to paper electrophoresis fro the detection of opine-positive clones which were considered to have been transformed. Mannopine and agropine were presented in hairy root clones while mannopine was presented in two hairy root clones. Eight hairy root clones were selected and cultured in MS, B5 and WP media. Each of hairy root clones was showed a difference in branch pattern and growth rate. The best culture medium and culture conditions of hairy roots were in MS(3% sucrose, pH 5.7) liquid medium at 25℃, 70 rpm under dark, the growth rate in MS liquid medium was increased with 210-fold more than that of inoculated hairy roots and with 2-fold more than that in MS liquid medium. Also, the adequate condition for hairy root growth was such that concentration of KH$2PO$4 was 1.25mM and the ratio of NH4 : NO was 1 to 3 in MS medium. The presence of pyrrolizidine alkaloids, monocrotaline, in the hairy roots was detected by TLC.

목차

서론
 재료 및 방법
  식물 재료 및 균주
  모상근의 유도 및 배양
  모상근 선발 및 형질전환 확인
  배양조건에 따른 생장률 측정
  Monocrotaline 분석
 결과 및 고찰
  모상근 유도 및 배양
  모상근의 선발 및 형질전환의 확인
  배양조건에 따른 생장률 측정
  Monocrotaline 분석
 요약
 감사
 참고문헌

키워드

저자

• 김영준 [ Young-Jun Kim | 전남대학교 생물학과 ]
• 표병식 [ Byoung-Sik Pyo | 동신대학교 식품생물공학과 ]
• 김광수 [ Kwang-Soo Kim | 전남대학교 생물학과 ]
• 황백 [ Baik Hwang | 전남대학교 생물학과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576