Earticle

초록

영어

Using rlFN- and rhGH as the model proteins, the refolding performances of the published processes were evaluated and compared. Key engineering parameters such as the type of denaturant and this concentration, protein concentration in the refolding buffer, and pH and ionic strength of the buffer were experimentally investigated. Furthermore, the role of a co-solvent of surfactant type in aggregation reduction was also studied. Of the denaturants tested (8M urea, 6M guanidine HCI, 0.5% SDS), SDS at alkaline pH (9.5) and ambient temperature gave the highest recovery yield. The SDS process was effective in the refolding of observed where dissolution proceeded better under lower strength (10 mM) but aggregation was suppressed under higher strength (>50 mM.) When PEG-4000 and/or Tween were added as co-solvent or refolding-enhancing additive, 1.6-2 times higher yield was realized. The‘masking’of the hyrophobic patches located on the surface of the protein with the surfactant molecules was believed to be responsible for the considerable reduction in aggregation during refolding.

목차

서론
 실험재료 및 방법
 결과 및 고찰
  산화 및 환원된 형태의 분리
  변성제의 내표체 용해성능 평가
  재접힘공정중 변성제 및 단백질 농도의 영향
  재접힘에 대한 이온 강도의 효과
  재접힘 첨가제의 영향
  재접힘공정중 응집현상
  음이온 교환수지를 이용한 SDS의 제거
  용해 및 재접힘공정의 회수율
 결론
 감사
 참고문헌

키워드

저자

• 김창성 [ Chang Sung Kim | 한양대학교 화학공학과 생물공정연구실 ]
• 김윤하 [ Yun Ha Kim | 쌍용정유 기술개발팀 ]
• 이은규 [ Eun Kyu Lee | 한양대학교 화학공학과 생물공정연구실 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576