Earticle

초록

영어

The present work proposes a simple electrochemical method applicable to any immobilization processes of oxidase using a Clark type oxygen electrode as a base transducer. The present work suggests an optimal immobilization technique among three different methods of glucose oxidase(GOD) onto one side of $37[u$]mthick blend membranes, composed o 80% of cellulose triacetate and 20% of polycaprolactone, on the basis of the maximum Michaelis-Menten parameter(Vm) determined by either steady state or transient analyses. The electrode system was made of disk type gold cathode(4mm diameter) and Ag/AgCl anode. One side of the blend membrane was in contact with the cathode surface while the other side was immobilized with GOD either in covalent-bond or cross-linked forms, the latter being covered by 25um thick dialysis membrane of cellulose acetate. The resultant current density was on-line monitored by a potentiostat while glucose level was varied from 1 to 20 mM. The present study shows that direct cross-linking of GOD with glutaraldehyde was mostly preferred for fabrication of glucose sensor, on the basis of resultant kinetic parameters from either steady state or transient analyses.

목차

ABSTRACT
 서론
 재료 및 방법
  실험장치
  전극
  효소 고정화
 결과 및 고찰
  Data 해석
  Kinetic Parameters
 결론
 감사의 글
 용어설명
 참고문헌

키워드

저자

• 정태훈 [ Tae-Hoon Jurng | 수원대학교 화학공학과 ]
• 홍석인 [ Suk-In Hong | 수원대학교 화학공학과 ]
• 노봉수 [ Bong-Soo Noh | 서울여자대학교 식품·미생물공학과 ]
• 정용섭 [ Yong-Seob Jeong | 전북대학교 식품공학과 ]
• 윤정원 [ Jeong-Weon Yoon | 수원대학교 유전공학과 ]
• 김태진 [ Tai-Jin Kim | 수원대학교 화학공학과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576