다른 결정성을 갖는 Cellulose에 대한 Cellulase의 비이온성 계면활성제 용액에서의 흡착거동
Adsorption Behaviors of Cellulose on Cellulose with Different Crystallinities in Nonionic Surfactant Solution
The adsorption of cellulase on celluloses with different crystallinities was carried out In nonionic surfactant(Tween 20) solution. Highly crystallized celluloses were prepared by enzymatic prehydrolyzation. From the experiments, the Langmuir isotherm parameters, maximum adsorption amount (Amax) and adsorption equilibrium constant(Kad) for the adsorption, were obtained in the presence and absence of nonionic surfactant. It was found that the Kad values were decreased by adding Tween 20. This indicates that the adsorption affinity is reduced by nonionic surfactant, and Amax decreased with increasing crystallinity under conditions accompanying in both the presence and absence of surfactant. The thermodynamic parameters such as ΔHa, ΔGa, and ΔSa for the adsorption were calculated by using the experimental data. From these results, it was found that the adsorption processes are exothermic reactions in both the presence and absence of surfactant. The heats of adsorption in surfactant solution(-4.68∼-3.62KJmol-1) are smaller than that of the adsorption in the absence of surfactant(-15.60∼-12.10KJmol-1). These results indicated that the tightness of adsorption was reduced by the addition of surfactant. The ΔSa values were estimated to be positive. This may suggest that the water and solute are released from cellulose on adsorption. The ΔSa values in surfactant solution are larger than that of the adsorption in the absence of surfactant. This may suggest that the binding of surfactant on hydrophobic region of cellulase cause dispersion of water and solute molecule orienting around the enzyme molecule. The surfactant played an important role in the desorption of enzyme from cellulose functional groups, and enhance the saccharification of the cellulose.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Materials Preparation of prehydrolyzed celluloses Determination of correlation crystallinity index. Adsorption of cellulase on celluloses with different crystallinities. RESULTS AND DISCUSSION Adsorption behaviors of cellulase on microcrys-talline celluloses. Thermodynamic properties of cellulase adsorption. 요약 REFERENCES
저자
김동원 [ Dong Won Kim | 충북대학교 자연과학대학 화학과 ]
Corresponding Author
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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