Earticle

초록

영어

Mineral salts in medium usually profoundly influence microorganism growth and protein synthesis. In order to produce microbial transglutaminase (MTG) with a high yield from Streptoverticillium mobaraense, we screened the minerals NaCl, CaCl2, CoCl2, FeSO4, ZnSO4, MnSO4 and CuSO4 for MTG fermentation. The results indicated that appropriate FeSO4
concentrations could significantly promote cell growth and stimulate the production of MTG. With 15 mg/L of FeSO4 added to medium, 58% improvements were noted in MTG productivity (2.24 U/mL). NaCl, CaCl2, and CoCl2 enhanced MTG productivity by less than 15%, and the optimal concentrations were determined as 1 g/L, 2 g/L, and 30 mg/L respectively.
Furthermore, it was determined that 7.5 mg/L of ZnSO4 in medium could augment MTG productivity by 20% and induce the stationary phase for MTG production to a period 24 hr earlier. This basic and novel discovery should result in the development of a good complement to the previously defined culture media for MTG fermentation.

목차

Abstract
 NTRODUCTION
 MATERIALS AND METHODS
  Microorganisms
  Culture methods
  Biomass dry weight
  Enzyme assay
  Measurement of residual sugar
  Dialysis assay
 RESULTS AND DISCUSSIONS
  Effect of tested minerals on MTG activity
  Effect of NaCl, CaCl2, CoCl2, FeSO4 on cell growthand MTG productivity
  Effect of MnSO4, CuSO4 and ZnSO4 on cell growthand MTG productivity
  Comparison of improved DCW and MTG productivityby the tested minerals
  Determination of the final concentrations of mineralsin medium
 CONCLUSION
 REFERENCES

키워드

저자

• Hong-Wei Wang [ Department of Bioscience and Biotechnology, College of Engineering ]
• In Hae Kim [ Department of Pharmaceutical Engineering, College of Medical Life Science Silla University ]
• Chang-Su Park [ Department of Pharmaceutical Engineering, College of Medical Life Science Silla University ]
• Jae-Hwa Lee [ Department of Bioscience and Biotechnology, College of Engineering, Department of Pharmaceutical Engineering, College of Medical Life Science Silla University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576