Earticle

초록

영어

Transformed rice plantlet were recovered from protoplasts by electroporation with the plasmld pB 1121, which contain the plant expressible NPT-II and GUS genes. Embryonic cell suspension culture was established with embryonic callus induced from mature seeds of rice (Oryza sativa L. cv. Dong-jin) on the MS medium supplemented with 2.0 mg/l 2,4-D, 0.5 mg/l kinetin, 3% sucrose. Protoplasts isolated from embryonic cell suspensions were electroplated and then poterltialty-transformed tissues were selected by growth on the medium containing 200 mg/l kanamycin sulfate. When subjected to GUS assay, they stained blue, indicating the expression of the inserted GUS genes. Plantlets were regenerated from electroplated protoplasts on the hormone free MS medium. Transferred foreign genes in the plants were confirmed by southern hybridization. These results support use of electroporation for transformation of these important cereal plants.

목차

ABSTRACT
 서론
 재료 및 방법
  배 발생 캘러스 유도 및 배양
  배 발생세포의 현탁배양
  사용균주 및 Plasmid DNA
  Plasmid DNA의 분리
  원형질체 분리 및 electroporation
  원형질체 배양 및 재분화
  형질전환 확인
 결과 및 고찰
 적요
 감사
 참고문헌

저자

• 황백 [ Baik Hwang | 전남대학교 생물학과 ]
• 황성진 [ Sung-JIn Hwng | 전남대학교 생물학과 ]
• 임형탁 [ Hyong Tak Im | 전남대학교 생물학과 ]
• 강영희 [ Young Hee Kang | 연세대학교 생물학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576