Earticle

초록

영어

In this paper the effect of light on non-aerated Baker's Yeast(Saccharomyces cereuisiae) production and the protein excretion to the extracellular fluid is studied. Previous results in our laboratory indicate that at pH=5 and T-32℃ yeast may be affected by light, but those differences seem to be within statistical variation of the data. In this paper, cell and extracellular protein concentrations along with redox potential are monitored for batch fermentations in the presence and absence of light at pH levels of 3 and 5 and at 31℃, in order to explore whether possible light effects can be more readily discerned at lower pH values. Yeast particle size distributions are also determined over the course of fermentation using a particle counter in order to add one more measuring tool to our usual cell and total protein measurements. An apparently noticeable difference in the redox potential is observed between the light and the dark runs for early times for the pH=3 runs. The particle size distributions show differences in the particle diameters between light and dark runs at pH=3, but those differences fall within one standard deviation of the mean particle diameters.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Microorganism
  Medium
  Apparatus
  Experimental Procedure
  Cell Concentration Measurement
  Total Extracellular Protein Measurement-The Bradford Assay
  Size Distribution
 RESULTS AND DISCUSSION
  Cell Concentration
  Protein Concentrations
  Redox Potential
  Size Distribution
 CONCLUSIONS AND RECOMMENDATIONS
 ACKNOWLEDGEMENT
 REFERENCES

저자

• Dowd Jr, Christopher J. [ Vanderbilt University ]
• Tanner, Robert D. [ Vanderbilt University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576