Improvement of Process for Sorbitol Production with Cetyltrimethylammoniumbromide Permeabilized Cells of Zymomonas mobilis through Glutaraldehyde Crosslinking
Cetyltrimethylammoniumbromide로 투과성을 높힌 Zymomonas mobilis의 Glutaraldehyde Crosslinking에 의한 Sorbitol 생산 안정성의 향상
Permeabilization of Zymomonas mobilis with CTAB(Cetyltrimethylammoniumbromide) was investigated in order to obtain stable process for sorbitol production in the immobilized system. The optimum conditions for sorbitol formation were obtained in the case of using cells treated with 0.2% CTAB at 4℃for 10 min. Permeabilized cells were treated with glutaraldehyde to cross-link the internal enzyme for the improvement of the enzyme stability. In this way, no significant loss of enzyme activity was apparent during 30-day operation in a continuous process. The productivity of the continuous process at dilution rate 0.2h-1 was 6.51g/1/h for sorbitol. The CTAB permeabilized cells could be used to produce sorbitol in the long term continuous process
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Microorganism and growth conditions Preparation of permeabilized cells and glutaraidehyde cross-linking Procedure for cell immobilization Reactor operation Analytical methods RESULTS AND DISCUSSION Sorbitol formation with toluene trated cells Sorbitol production with CTAB treated cells Stability of continuous process 요약 ACKNOWLEDGEMENTS REFERENCES
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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