Methylobacterium sp. GL-10의 유가식 배양에 의한 Methanol로 부터 Poly-B-hydroxybutyrate의 생산
Production of Poly-B-hydroxybutyrate from Methanol by Fed-batch Cultivation of methylobacterium sp. GL-10
The production of poly-B-hydroxybutyrate(PHB) from methanol by batch and fed-batch cultivations of Methylobacterium sp. GL-10 was studied. PHB accumulation was stimulated by the nutrients deficiency including, NH4+, SO42-, and K+. The nitrogen deficiency was the most critical factor for PHB accumulation. In batch cultivation, the maximum cell concentration and PHB content were 1.86g/l and 0.62g/l, respectively, with 1.0%(v/v) of methanol and 0.5g/1 of ammonium sulfate. The mass doubling time of Methylobacterum sp. GL-10 was in the range of 4-5 hrs. The cell growth and PHB accumulation were severely inhibited at the methanol concentration over than 2% (v/v). To overcome methanol Inhibition, constant feeding and intermittent feedillg fed-batch cultivations were adopted, using C/N molar ratio as a control factor. In constant feeding fed-batch process, cell concentration was increased up to 2.67g/1, and PHB yield was enhanced from 0.33 of batch culture to 0.53. The relatively low cell concentration was caused by methanol accumulated in culture broth at late growth phase. To prevent methanol accumulation and to maximize PHB production, DO-state intermittent fed-batch cultivation was attempted. The cell and PHB concentration was reached up to 4.55g/1 and 1.80g/1, respectively. It was possible to maintain methanol concentration low and also to feed nutrient of desired C/N molar ratio.
목차
ABSTRACT 서론 재료 및 방법 균주 및 배지조성 2단계 배양(Two-stage cultivation) 회분식 배양(Batch cultivation) Constant feeding fed-batch cultivation DO-stat intermittent feeding fed-batch cultivation 메탄올 농도의 측정 암모늄 이온 농도의 측정 균체농도의 측정 PHB의 추출 및 정량 결과 및 고찰 Methylobacterium sp. GL-10균주의 균체생육 및 PHB생합성 특성 균체생육과 PHB 축적에 미치는 각종 영양소원 결핍의 영향 각종 영양소원의 농도에 따른 PHB 축적효과 질소원의 농도 기타 영양소원의 농도 메탄올 농도 Constant feeding fed-batch cultivation DO-stat intermittent feeding fed-batch cultivation 요약 참고문헌
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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