Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli: PartIV. Effects of Ampicillin and an Inducer on the Production of Alpha-Interferon and Plasmid Stability
유전자 재조합 대장균을 사용한 Alpha-interferon의 생산과 분비;제4부. Ampicillin 및 Inducer의 Alpha-interferon의 생산과 Plasmid 안정성에 미치는 영향
We studied the production and excretion of alpha-interferon in recombinant Escherichia coli harboring plasmid pIF-III-B, which carries alpha-interferon gene under the control of lipoprotein and lacUV5 promoter, and lac operator. Basically, the effects of concentrations of ampicillin and an inducer, IPTG, for the expression of the cloned gene, on the productions of alpha-interferon and plasmid stability were studied. The highest production of alpha-interferon was observed at 50 mg/1 of ampicillin concentration and 0.5 mM of IPTG. The plasmid pIF-III-B was maintained very stably in medium with ampicillin but segregated rapidly in medium without ampicillin. Also, the plasmid was segregated more rapidly in medium with an inducer higher than 0.5 mM.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Bacterial strains, plasmid and culture conditions Determination of alpha-IFN activity Preparation of samples for the assay of alpha-IFN Determination of plasmid stability RESULTS AND DISCUSSION Effect of ampicilin concentration on the production of alpha-IFN Effect of IPTG concentration on the production of aiPha-lFN Plasmid stability ABBREVIATIONS 요약 REFERENCES
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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