Flow cytometry offers quick and statistically reliable information about the physiological state of cells. In this study, the inactivation of Salmonella typhimurium treated at the various temperature (35-55°C at 100 bar) and pressure (80-250 bar at 35°C) by supercritical carbon dioxide (SC-CO2) was analyzed by the flow cytometry. Using fluorescent dyes such as propidium iodide (PI) and ethidium bromide (EB), which distinguish the physiological state by specific dyes indicating the membrane integrity and the functionality of efflux pump, respectively, the state of S. typhimurium treated with SC-CO2 was analyzed. By the combined staining with SYTO 9 and PI, there was a remarkable decrease in the of membrane integrity as increasing the SC-CO2 treatment. The staining with SYTO 9 and EB staining revealed that the efflux pump system was damaged even at a less severe condition compared to the membrane integrity.
저자
Hee Taek Kim [ Division of Food Bioscience and Technology College of Life Sciences and Biotechnology Korea University ]
Soo Rin Kim [ Division of Food Bioscience and Technology College of Life Sciences and Biotechnology Korea University ]
Kyoung Heon Kim [ Division of Food Bioscience and Technology College of Life Sciences and Biotechnology Korea University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동