Earticle

초록

영어

This paper describes the role of nutrients in the production of rifamycin B by Amycolatopsis mediterranei S699 in optimal medium. The central composite design experiments showed that optimal concentrations of glucose and ammonium sulfate were 63 g/L and 7.9 g/L at 10 g/l of corn steep liquor (CSL). A bioreactor culture was performed in optimal medium. The total amino acids derived from CSL were completely depleted and 50% of the ammonium ion was used in lag phase. Cell growth was concomitant with uptake glucose and inorganic phosphate and ceased due to depletion of inorganic phosphate. Rifamycin B production began when the cell growth started, however, its production rate increased after the cell growth stopped. During this period, protein derived from CSL hydrolyzed to free amino acids which were then utilized and
simultaneous utilization of ammonium ions was observed. Production stopped at 172 h when the glucose was depleted. The maximum cell concentration and rifamycin B titer were 8.6 g/L and 1260 mg/L, respectively. This result implied that glucose and ammonium sulfate contributed to cell growth and product formation whereas CSL was found to enhance the production rate and increasing rifamycin B titer.

저자

• Feng Xu Ma [ Department of Chemical & Biological Engineering, GyeongSang National University ]
• Jung Hun Kim [ Department of Chemical & Biological Engineering, GyeongSang National University ]
• Sung Bae Kim [ Department of Chemical & Biological Engineering, GyeongSang National University ]
• He Li [ Department of Chemical & Biological Engineering, GyeongSang National University ]
• Chang-Joon Kim [ Department of Chemical & Biological Engineering, GyeongSang National University ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576