Earticle

초록

영어

Regulons, sub-parts of functional transcriptional regulatory network(TRN) that are regulated upon a specific stimulus, have been extensively studied and known to consist of transcription factors(TFs) and their target genes. However, most of their dynamic properties – what regulons are really reacting to each stimulus – have not been clearly concluded yet even in microorganisms. In this study, 3 genotoxicants, AgNO3 and Ag-nanoparticles(10nm size) were used to construct the functional TRN in E. coli. First, the significantly perturbed TFs upon each stress were selected from microarray anaylsis referring to DBD and RegulonDB. To check whether those TFs are really responsible with each stress, fitness test was done for each TF deletion strain. For the selected TFs which were shown as essential for cell’s survival upon each stress, their latent target genes were found using microarray with deletion strains. Integrating the results with the prior static TRN from regulonDB, some regulons which have not been expected well were suggested. The result shows that genotoxic stress and metal induced stress have very distinct functional TRNs and some putative TFs are highly confirmative for their relevance on certain stress responses in E. coli.

저자

• Jung Eui Hong [ Graduate School of Life Sciences & Biotechnology, Korea University, ]
• Joo Myung Ahn [ Graduate School of Life Sciences & Biotechnology, Korea University, ]
• Byoung Chan Kim [ Institut Pasteur Korea ]
• Man-Bock Gu [ Graduate School of Life Sciences & Biotechnology, Korea University ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576