Earticle

초록

영어

The diversity of marine bacterial communities has been extensively investigated in many studies using the traditional culture methods. Such techniques are known for their selectivity and are not considered representative of the extent of the bacterial community. The proportion of cells which can be cultured is estimated to be 0.1% of the total population and few data are available
concerning how closely they reflect the actual composition of these communities. Comparison of the base sequences of 16S rRNA has become a powerful tool to infer the evolutionary pathways of procaryotes. Recently, a procedure that uses 16S rRNA sequence homology was proposed for defining and enumerating the components of a mixed natural population of bacteria.. It should allow the definition of a population, both quantitatively (species diversity) and qualitatively
(phylogenetic relatedness), without culturing1). In this study, we investigated the bacterial communities associated with the marine water in the East Sea. Marine water samples were collected in Damupo, Pohang, Korea and the environmental DNA was directly extracted from the sea water. Bacterial 16S rRNA gene library of 200 clones was generated using bacterial universal primers and 16S rDNA sequences were analyzed phylogenetically. The library was dominated by
marine uncultured bacteria, gamma-Proteobacteria, and alpha-Proteobacteria. Ninety-four percent of the cloned sequences was highly related to the known bacteria (Rhodobacter, Bacillus, Acidobacteria), while some of the cloned sequences showed less affiliation with known taxa (< 90% sequence similarity) and may represent novel taxa.

저자

• Sun Ja Cho [ Department of Chemical Engineering, Pohang University of Science & Technology ]
• Sun Bok Lee [ Department of Chemical Engineering, Pohang University of Science & Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576