Bo-Hwa Lee, Bo-Kyung, Kim, Hye-Jin Kim, Chung-Han Chung, Soo-Wan Nam, Sung-Koo Kim, Jin-Woo Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A100283
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원문정보
초록
영어
Bacillus subtilis subsp. subtilis A-53 was isolated from the seashore of the Kyungsang province in Korea.1) The highest production of cellulase was obtained when concentrations of rice hull and yeast extract were 5.0% (w/v) and 0.0% (w/v). Optimal initial pH of the medium and temperature were 6.8 and 37℃. Under these conditions, optimal agitation speed and aeration rate in a 7L bioreactor were found to be 400 rpm and 1.0 vvm.2) The gene coding cellulase in B. subtilis subsp. subtilis A-53 was isolated and cloned. The deduced amino acid sequence of the cellulase showed high identity to cellulase from other Bacillus species. E. coli JM109 was used as a host strain for cloning and maintenance of plasmid. The production of cellulase by B. subtilis subsp. subtilis A-53 and its transformant from rice bran as a carbon source was 68.7(U/ml) and 868.8(U/ml),respectively.
저자
Bo-Hwa Lee [ Division of Applied Biotechnology, Dong-A University ]
Bo-Kyung, Kim [ Division of Applied Biotechnology, Dong-A University ]
Hye-Jin Kim [ Division of Applied Biotechnology, Dong-A University ]
Chung-Han Chung [ Division of Applied Biotechnology, Dong-A University ]
Soo-Wan Nam [ Department of Biotechnology & Bioengineering, Dong-Eui University ]
Sung-Koo Kim [ Department of Biotechnology & Bioengineering, Pukyung National University ]
Jin-Woo Lee [ Division of Applied Biotechnology, Dong-A University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동