Due to dwindling of fossil fuel, microbial production of bio-fuel from organic byproducts has acquired significance in recent years. Ethanol has been trusted as an alternate fuel for the future. Zymomonas mobilis ferments sugar into ethanol with two different enzymes, alcohol dehydrogenase and pyruvate decarboxylase.2) By inserting Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II in E. coli, E. coli was able to ferment sugars into ethanol.1,3) This study Zymomonas mobilis (containing pdc and adhB genes) were used as the construction sources of genes and plasmids. Expression vectors carrying pdc and adhB genes were constructed by using pET-32a vectors. Ethanol productivity in Escherichia coli strain seemed to be affected by the extent of expresstion of pdc gene along with adhB genes. By successful gene mutation we could establish a new Escherichia coli strain which can produce ethanol efficiently.
저자
Eunkyoung Oh [ Department of Chemical and Biomolecular Engineering, Sogang University ]
Jinwon Lee [ Department of Chemical and Biomolecular Engineering, Sogang University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동