Antimicrobial peptides (AMPs) are agents of the most ancient form of defense systems. With the increasing concerns about the antibiotics- resistant bacteria, the AMPs have attracted attention as alternative antibacterial agents. To date, however, few effective high-throughput approaches have been developed for designing and screening peptides. In this work, we attempted to express biologically active antimicrobial peptides in a cell-free protein synthesis system. Through numerous approaches to enhance the efficiency of translation as well as to increase the stability of the expressed peptides, preparative amounts of active antimicrobial peptides were successfully expressed in the developed system.
키워드
cell-free protein synthesisantimicrobial peptideantibiotics
저자
Kyung-Ho Lee [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
Jin-Ho Ahn [ Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University ]
Dong-Myung Kim [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
Corresponding author
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동