The pYES2/CT and pYES3/CT, yeast expression vectors, are used for 1,2-propanediol production. Maximum β-galactosidase specific activity was assayed using the pYES2/CT/lacZ and pYES3/CT/lacZ for determination of harvest time. The pYES2/CT and pYES3/CT are 6.0 kb and 5.9 kb vector, respectively. The vectors contain the 2μ origin for maintenance and replication in yeast1). The pYES2/CT and pYES3/CT contain URA3 gene and TRP1 gene marker for selection, respectively. The vectors contain the GAL1 promoter. The GAL1 promoter require addition of galactose for induce expression of protein2). The effect of medium composition, glucose and galactose concentration on LacZ gene expression level were investigated. Assay of β-galactosidase specific activity study performed with Saccharomyces cerevisiae KCCM 50515. β -galactosidase assay was performed by ο-nitrophenyl-β-D-galactopyranoside (ONPG)3). Maximum β-galactosidase specific activity was obtained after 41 h of growth in YPDG medium containing 1.5% glucose and 0.5% galactose.
저자
So Young Kim [ Department of Biological Engineering, Inha University ]
Hyun Shik Yun [ Department of Biological Engineering, Inha University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
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